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For the purposes of estimation of appropriate sample size in rat, this study focused difference between sample mean and population mean at different three probabilities(α=0.03, 0.05, 0.07) for the observation of body weight, serum contents and inner organ weight. Through Tchebysheff’s theory(law of large numbers), sample size at α=0.07 was estimate at least 8 heads for body weight, 12 heads for serum Contents and 15 heads for inner organ weight. at α=0.05, sample size will be required 21 heads for body weight, 32 heads for serum contents and 42 heads for inner organ weight. For the probability 97%(α=0.03) sample size for accurate statistical analysis should be provided 98 heads for body weight, 138 heads for serum contents and 192 heads for inner organ weight. Generally statistical analysis for initial birth weight and serum Ca are required less experimental numbers, while serum Mg, serum Hydroxyproline and inner organ weight are more experimental numbers.
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